Testing Standard
Testing Standard
Testing Standard
With the purpose of protecting Athletes
With the purpose of protecting Athletes
With the purpose of protecting Athletes
Understanding the Clean Sport Certification
The Clean Sport Certification verifies that supplements have undergone thorough testing for anabolic androgenic steroids and stimulants prohibited by WADA. While it is impossible to guarantee that a product is entirely free of doping substances, our certification signifies that the product has been independently tested and controlled. As a result, the risk of contamination and the presence of banned substances is significantly minimized. Anabolic-androgenic steroids have been shown to be the most common family of molecules in cases of contamination of supplements from doping related substances. Stimulants have been shown to be an increasingly common contaminant in supplements.
The analyses therefore of these two families of compounds provides currently the best safety an athlete can expect.
Testing Phases:
1-3. All the testing parameters of the Certified Contents Certification
4. Testing for Anabolic Androgenic Steroids and Stimulants
Understanding the Clean Sport Certification
The Clean Sport Certification verifies that supplements have undergone thorough testing for anabolic androgenic steroids and stimulants prohibited by WADA. While it is impossible to guarantee that a product is entirely free of doping substances, our certification signifies that the product has been independently tested and controlled. As a result, the risk of contamination and the presence of banned substances is significantly minimized. Anabolic-androgenic steroids have been shown to be the most common family of molecules in cases of contamination of supplements from doping related substances. Stimulants have been shown to be an increasingly common contaminant in supplements.
The analyses therefore of these two families of compounds provides currently the best safety an athlete can expect.
Testing Phases:
1-3. All the testing parameters of the Certified Contents Certification
4. Testing for Anabolic Androgenic Steroids and Stimulants
The Clean Sport Certification verifies that supplements have undergone thorough testing for anabolic androgenic steroids and stimulants prohibited by WADA. While it is impossible to guarantee that a product is entirely free of doping substances, our certification signifies that the product has been independently tested and controlled. As a result, the risk of contamination and the presence of banned substances is significantly minimized. Anabolic-androgenic steroids have been shown to be the most common family of molecules in cases of contamination of supplements from doping related substances. Stimulants have been shown to be an increasingly common contaminant in supplements.
The analyses therefore of these two families of compounds provides currently the best safety an athlete can expect.
Testing Phases:
1-3. All the testing parameters of the Certified Contents Certification
4. Testing for Anabolic Androgenic Steroids and Stimulants
PHASE 1
Ingredient Value Accuracy
In this first phase we identify and measure the labelled bio-active compounds of your supplements. We focus on Vitamins, Essential Minerals, Fish Oils (Omega 3 and Omega 6), Proteins, Probiotics as well as Amino Acids. We also verify the nutritional values of the product including Calories, Carbohydrates, Salt and Fats where relevant.
PHASE 1
Ingredient Value Accuracy
In this first phase we identify and measure the labelled bio-active compounds of your supplements. We focus on Vitamins, Essential Minerals, Fish Oils (Omega 3 and Omega 6), Proteins, Probiotics as well as Amino Acids. We also verify the nutritional values of the product including Calories, Carbohydrates, Salt and Fats where relevant.
PHASE 1
Ingredient Value Accuracy
In this first phase we identify and measure the labelled bio-active compounds of your supplements. We focus on Vitamins, Essential Minerals, Fish Oils (Omega 3 and Omega 6), Proteins, Probiotics as well as Amino Acids. We also verify the nutritional values of the product including Calories, Carbohydrates, Salt and Fats where relevant.
To do this we employ analytical methodologies from US Pharmacopeia (USP), European Pharmacopeia (EP) as well as in-house methods and use state of the art instrumentation, including but not limited to:
To do this we employ analytical methodologies from US Pharmacopeia (USP), European Pharmacopeia (EP) as well as in-house methods and use state of the art instrumentation, including but not limited to:
To do this we employ analytical methodologies from US Pharmacopeia (USP), European Pharmacopeia (EP) as well as in-house methods and use state of the art instrumentation, including but not limited to:
HPLC-UV
High Pressure Liquid Chromatography with Ultraviolet detector
HPLC-UV
High Pressure Liquid Chromatography with Ultraviolet detector
HPLC-UV
High Pressure Liquid Chromatography with Ultraviolet detector
HPLC-UV
Ultra Performance Liquid Chromatography couple with Double Quadrupole Mass Spectrometer
HPLC-UV
Ultra Performance Liquid Chromatography couple with Double Quadrupole Mass Spectrometer
HPLC-UV
Ultra Performance Liquid Chromatography couple with Double Quadrupole Mass Spectrometer
HPLC-MS
High Pressure Liquid Chromatography coupled with Mass Spectrometer
HPLC-MS
High Pressure Liquid Chromatography coupled with Mass Spectrometer
HPLC-MS
High Pressure Liquid Chromatography coupled with Mass Spectrometer
FTIR
Fourier Transform Infrared Spectroscopy
FTIR
Fourier Transform Infrared Spectroscopy
FTIR
Fourier Transform Infrared Spectroscopy
ICP-MS
Ion Coupled Plasma coupled with Mass Spectrometer
ICP-MS
Ion Coupled Plasma coupled with Mass Spectrometer
ICP-MS
Ion Coupled Plasma coupled with Mass Spectrometer
GC
Gas Chromatography
GC
Gas Chromatography
GC
Gas Chromatography
GC-MS
Gas Chromatography coupled with Mass Spectrometer
GC-MS
Gas Chromatography coupled with Mass Spectrometer
GC-MS
Gas Chromatography coupled with Mass Spectrometer
PCR
Polymerase Chain Reaction
PCR
Polymerase Chain Reaction
PCR
Polymerase Chain Reaction
NGS
Next Generation Sequencing
NGS
Next Generation Sequencing
NGS
Next Generation Sequencing
ELISA
Enzyme-linked Immunosorbent Assay
ELISA
Enzyme-linked Immunosorbent Assay
ELISA
Enzyme-linked Immunosorbent Assay
Laboratory Benchwork of Compendial Methods
Laboratory Benchwork of Compendial Methods
Laboratory Benchwork of Compendial Methods
PHASE 2
Disintegration/Dissolution
In this second step we make sure that the supplement disintegrates in our bodies and therefore renders its bio-active compounds available for bodily uptake.
When you consume a supplement, it undergoes digestion in the gastrointestinal tract. The active ingredients are then absorbed into the bloodstream through the intestinal walls. From there, they enter systemic circulation, allowing them to be transported to various parts of the body where they can exert their effects.
The bioavailability, or the extent and rate at which the active ingredients are absorbed and become available at the site of action, can vary widely among different supplements. Factors influencing bioavailability include the supplement's formulation (such as whether it is a liquid, capsule, tablet, or powder), the presence of other components or nutrients, and individual factors like metabolic rate and gastrointestinal health.
Disintegration is thus a prerequisite for efficient nutrient availability and subsequent bodily uptake. Without it even high-quality products may fail to be absorbed, rendering the supplement inefficient.
Compliance with the limits on Disintegration stated in the individual monographs for supplements is required except for Delayed release (enteric coated tablets) and Timed or slow release claiming supplements where USP methodology will be followed using gastric fluid simulant or 0.1M HCl for a period no greater than 8 hours. In these slow or delayed release cases the supplements should not disintegrate within the first hour. Chewables, powders, and liquids are exempt from disintegration testing requirements.
Standard disintegration will follow USP and EP monographs where applicable. In case of no such monograph being present disintegration will be carried out over 60 min in deionized water.
In this step the instrumentation used is a Disintegration Tester.
PHASE 2
Disintegration/Dissolution
In this second step we make sure that the supplement disintegrates in our bodies and therefore renders its bio-active compounds available for bodily uptake.
When you consume a supplement, it undergoes digestion in the gastrointestinal tract. The active ingredients are then absorbed into the bloodstream through the intestinal walls. From there, they enter systemic circulation, allowing them to be transported to various parts of the body where they can exert their effects.
The bioavailability, or the extent and rate at which the active ingredients are absorbed and become available at the site of action, can vary widely among different supplements. Factors influencing bioavailability include the supplement's formulation (such as whether it is a liquid, capsule, tablet, or powder), the presence of other components or nutrients, and individual factors like metabolic rate and gastrointestinal health.
Disintegration is thus a prerequisite for efficient nutrient availability and subsequent bodily uptake. Without it even high-quality products may fail to be absorbed, rendering the supplement inefficient.
Compliance with the limits on Disintegration stated in the individual monographs for supplements is required except for Delayed release (enteric coated tablets) and Timed or slow release claiming supplements where USP methodology will be followed using gastric fluid simulant or 0.1M HCl for a period no greater than 8 hours. In these slow or delayed release cases the supplements should not disintegrate within the first hour. Chewables, powders, and liquids are exempt from disintegration testing requirements.
Standard disintegration will follow USP and EP monographs where applicable. In case of no such monograph being present disintegration will be carried out over 60 min in deionized water.
In this step the instrumentation used is a Disintegration Tester.
PHASE 2
Disintegration/Dissolution
In this second step we make sure that the supplement disintegrates in our bodies and therefore renders its bio-active compounds available for bodily uptake.
When you consume a supplement, it undergoes digestion in the gastrointestinal tract. The active ingredients are then absorbed into the bloodstream through the intestinal walls. From there, they enter systemic circulation, allowing them to be transported to various parts of the body where they can exert their effects.
The bioavailability, or the extent and rate at which the active ingredients are absorbed and become available at the site of action, can vary widely among different supplements. Factors influencing bioavailability include the supplement's formulation (such as whether it is a liquid, capsule, tablet, or powder), the presence of other components or nutrients, and individual factors like metabolic rate and gastrointestinal health.
Disintegration is thus a prerequisite for efficient nutrient availability and subsequent bodily uptake. Without it even high-quality products may fail to be absorbed, rendering the supplement inefficient.
Compliance with the limits on Disintegration stated in the individual monographs for supplements is required except for Delayed release (enteric coated tablets) and Timed or slow release claiming supplements where USP methodology will be followed using gastric fluid simulant or 0.1M HCl for a period no greater than 8 hours. In these slow or delayed release cases the supplements should not disintegrate within the first hour. Chewables, powders, and liquids are exempt from disintegration testing requirements.
Standard disintegration will follow USP and EP monographs where applicable. In case of no such monograph being present disintegration will be carried out over 60 min in deionized water.
In this step the instrumentation used is a Disintegration Tester.
PHASE 3
Impurities and Contaminants
Finally our third pillar of quality consists in verifying that the Food Supplement does not contain contaminants related to the raw materials used in its production. Here we assess the microbial sterility of the product and we test for the presence of heavy metals as well as other ingredient-specific contaminants and impurities.
We test for Heavy metals. (As, Pb, Hg, Cd) using ICP-MS instrumentation
We test the microbial purity of the formulation and particularly for Total Aerobic Microbial Count (TAMC), Total Yeasts and Molds Count (TYMC), Staphylococcus Aureus, E. Coli, Salmonella, Pseudomonas Aeruginosa, Bile-tolerant Gram-negative bacteria.
Contaminants can pose serious health risks even at low levels. Comprehensive screening ensures the safety and purity of supplements, protecting end-users from potential toxicities and supporting compliance with stringent regulatory standards.
PHASE 3
Impurities and Contaminants
Finally our third pillar of quality consists in verifying that the Food Supplement does not contain contaminants related to the raw materials used in its production. Here we assess the microbial sterility of the product and we test for the presence of heavy metals as well as other ingredient-specific contaminants and impurities.
We test for Heavy metals. (As, Pb, Hg, Cd) using ICP-MS instrumentation
We test the microbial purity of the formulation and particularly for Total Aerobic Microbial Count (TAMC), Total Yeasts and Molds Count (TYMC), Staphylococcus Aureus, E. Coli, Salmonella, Pseudomonas Aeruginosa, Bile-tolerant Gram-negative bacteria.
Contaminants can pose serious health risks even at low levels. Comprehensive screening ensures the safety and purity of supplements, protecting end-users from potential toxicities and supporting compliance with stringent regulatory standards.
PHASE 3
Impurities and Contaminants
Finally our third pillar of quality consists in verifying that the Food Supplement does not contain contaminants related to the raw materials used in its production. Here we assess the microbial sterility of the product and we test for the presence of heavy metals as well as other ingredient-specific contaminants and impurities.
We test for Heavy metals. (As, Pb, Hg, Cd) using ICP-MS instrumentation
We test the microbial purity of the formulation and particularly for Total Aerobic Microbial Count (TAMC), Total Yeasts and Molds Count (TYMC), Staphylococcus Aureus, E. Coli, Salmonella, Pseudomonas Aeruginosa, Bile-tolerant Gram-negative bacteria.
Contaminants can pose serious health risks even at low levels. Comprehensive screening ensures the safety and purity of supplements, protecting end-users from potential toxicities and supporting compliance with stringent regulatory standards.
PHASE 4
Anabolic-androgenic steroids + Stimulants
We have focused on these two major families of substances prohibited by WADA as they are the ones mostly associated with cases of doping contamination in food supplements. By employing LC-MS and GC-MS methodologies, we are able to descend to very low reporting levels of 10ng/g for anabolic androgenic steroids and 100ng/g for stimulants. By combining the analysis for stimulants with the one for anabolic-androgenic steroids at such reporting levels we are able to provide the best safety currently possible.
In this forth phase, we control for these two families of compounds provides providing currently the best safety an athlete can expect.
PHASE 4
Anabolic-androgenic steroids + Stimulants
We have focused on these two major families of substances prohibited by WADA as they are the ones mostly associated with cases of doping contamination in food supplements. By employing LC-MS and GC-MS methodologies, we are able to descend to very low reporting levels of 10ng/g for anabolic androgenic steroids and 100ng/g for stimulants. By combining the analysis for stimulants with the one for anabolic-androgenic steroids at such reporting levels we are able to provide the best safety currently possible.
In this forth phase, we control for these two families of compounds provides providing currently the best safety an athlete can expect.
PHASE 4
Anabolic-androgenic steroids + Stimulants
Anabolic-androgenic steroids have been shown to be the most common family of molecules in cases of contamination of supplements from doping relevant substances. Stimulants have been shown to be an increasingly common contaminant in supplements.
In this forth phase, we control for these two families of compounds provides currently the best safety an athlete can expect.